Three decades after it was conceived, the world's most powerful physics experiment is ready to be powered up.
Engineers will attempt to circulate a beam of particles around the 27km-long underground tunnel which houses the Large Hadron Collider (LHC).
The £5bn machine is designed to smash particles together with cataclysmic force, revealing signs of new physics in the wreckage.
This will re-create conditions in the Universe moments after the Big Bang.
But it has not been plain sailing; the project has been hit by cost overruns, equipment trouble and construction problems. The switch-on itself is two years late.
The collider is operated by the European Organization for Nuclear Research - better known by its French acronym Cern.
The vast circular tunnel - the "ring" - which runs under the French-Swiss border contains more than 1,000 cylindrical magnets arranged end-to-end.
The magnets are there to steer the beam - made up of particles called protons - around this 27km-long ring.
At allotted points around the tunnel, the beams will cross paths, smashing together near four massive "detectors" that monitor the collisions for interesting events.
Scientists are hoping that new sub-atomic particles will emerge, revealing fundamental insights into the nature of the cosmos.
"We will be able to see deeper into matter than ever before," said Dr Tara Shears, a particle physicist at the University of Liverpool.
"We will be looking at what the Universe was made of billionths of a second after the Big Bang. That is amazing, that really is fantastic."
The LHC should answer one very simple question: What is mass?
The currently favoured model involves a particle called the Higgs boson - dubbed the "God Particle". According to the theory, particles acquire their mass through interactions with an all-pervading field carried by the Higgs.
The latest astronomical observations suggest ordinary matter - such as the galaxies, gas, stars and planets - makes up just 4% of the Universe.
The rest is dark matter (23%) and dark energy (73%). Physicists think the LHC could provide clues about the nature of this mysterious "stuff".
Full beam ahead
Engineers injected the first low-intensity proton beams into the LHC in August. But they did not go all the way around the ring.
Now they will attempt to pass a proton beam around the full circumference of the LHC tunnel.
"We see how far the beam will go," said Steve Myers, head of the accelerator and beams department at Cern, "we will try and make it go round the full 27km sometime on Wednesday morning."
Engineers will be on the lookout for any potential problems: "There are on the order of 2,000 magnetic circuits in the machine. This means there are 2,000 power supplies which generate the current which flows in the coils of the magnets," he told BBC News.
"If any single one of them has got the wrong polarity, or has the wrong calibration constant, or whatever, then the beam will not go round.
"If, in any of the channels [in the magnets], there is any piece of debris - it is a very small channel - then the beam will not go round."
Mr Myers has experience of the latter problem. While working on the LHC's predecessor, a machine called the Large-Electron Positron Collider, engineers found two beer bottles wedged into the beam pipe - a deliberate, one-off act of sabotage.
If all goes well, and the beam makes one turn, engineers will "close the orbit", allowing the beam to circulate continuously around the LHC.
Engineers will then try to "capture" it. The beam which circles the LHC is not continuous; it is composed of several packets - each about a meter long - containing billions of protons.
The protons would disperse if left to their own devices, so engineers use electrical forces to "grab" them, keeping the particles tightly huddled in packets.
Once the beam has been captured, the same system of electrical forces is used to give the particles an energetic kick, accelerating them to greater and greater speeds.
After Wednesday's test, engineers will need to get two beams running in opposite directions around the LHC. They can then carry out collisions by smashing them together.
The idea of the Large Hadron Collider emerged in the early 1980s. The project was eventually approved in 1996 at a cost of SFr2.6bn.
However, Cern underestimated equipment and engineering costs when it set out its original budget, plunging the lab into a cash crisis.
Cern had to borrow hundreds of millions of euros in bank loans to get the LHC completed. The current price is nearly four times that originally envisaged.
During winter, the LHC will be shut down, allowing equipment to be fine-tuned for collisions at full energy.
"What's so exciting is that we haven't had a large new facility starting up for years," explained Dr Shears.
"Our experiments are so huge, so complex and so expensive that they don't come along very often. When they do, we get all the physics out of them that we can."
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